Structure and stability of non-symmetric Burgers vortices

We investigate, numerically and analytically, the structure and stability of steady and quasi-steady solutions of the Navier–Stokes equations corresponding to stretched vortices embedded in a uniform non-symmetric straining field, ([alpha]x, [beta]y, [gamma]z), [alpha]+[beta]+[gamma]=0, one principal axis of extensional strain of which is aligned with the vorticity. These are known as non-symmetric Burgers vortices (Robinson & Saffman 1984). We consider vortex Reynolds numbers R=[Gamma]/(2[pi]v) where [Gamma] is the vortex circulation and v the kinematic viscosity, in the range R=1[minus sign]104, and a broad range of strain ratios [lambda]=([beta][minus sign][alpha])/([beta]+[alpha]) including [lambda]>1, and in some cases [lambda][dbl greater-than sign]1. A pseudo-spectral method is used to obtain numerical solutions corresponding to steady and quasi-steady vortex states over our whole (R, [lambda]) parameter space including [lambda] where arguments proposed by Moffatt, Kida & Ohkitani (1994) demonstrate the non-existence of strictly steady solutions. When [lambda][dbl greater-than sign]1, R[dbl greater-than sign]1 and [epsilon][identical with][lambda]/R[double less-than sign]1, we find an accurate asymptotic form for the vorticity in a region 11. An iterative technique based on the power method is used to estimate the largest eigenvalues for the non-symmetric case [lambda]>0. Stability is found for 0[less-than-or-eq, slant][lambda][less-than-or-eq, slant]1, and a neutrally convective mode of instability is found and analysed for [lambda]>1. Our general conclusion is that the generalized non-symmetric Burgers vortex is unconditionally stable to two-dimensional disturbances for all R, 0[less-than-or-eq, slant][lambda][less-than-or-eq, slant]1, and that when [lambda]>1, the vortex will decay only through exponentially slow leakage of vorticity, indicating extreme robustness in this case

Licenciamento Ambiental em Pequenas Propriedades Suinícolas: Um Estudo de Caso no Município de Braço do Norte-SC

Artigo Publicado em: VII Simpósio Internacional de Qualidade AmbientalO estado de Santa Catarina é o maior produtor de suínos, contando com um efetivo com cerca de 7,9 milhões de cabeças, representando uma importante atividade econômica para o país. Contudo, essa atividade gera fortes impactos ambientais, principalmente pela grande quantidade de efluentes, que se não tratados de forma adequada podem contaminar os recursos naturais, tanto o solo como os corpos d’água e o ar. Por ser uma atividade potencialmente poluidora, o licenciamento ambiental é previsto em lei e sua fiscalização tem sido crescente. Deste modo, o objetivo deste trabalho é elucidar o processo de licenciamento ambiental em pequenas propriedades rurais onde não é viável a adequação ao código florestal. Para isso utilizou-se um estudo de caso feito a partir do acompanhamento de um Termo de Ajustamento de Conduta (TAC) firmado entre o MP-SC e o suinocultor. Como principais medidas compensatórias e mitigatórias, o TAC propõe um projeto técnico para a adequação das estruturas edificadas, averbação de reserva legal além dos 20% exigido por lei e um plano para recuperação de áreas degradadas (PRAD) nas regiões de APP passiveis de recuperação. Conclui-se que com a implantação das medidas solicitadas haverá uma grande redução do impacto ambiental causado pela propriedade suinícola, contudo o processo de licenciamento exigido é bastante burocrático e oneroso tornando-se distante da realidade dos suinocultores, principalmente para as pequenas propriedades com mão de obra familiar

EFSUMB Recommendations and Guidelines for Gastrointestinal Ultrasound - Part 1: Examination Techniques and Normal Findings (Short version)

Abstract ▼ In October 2014 the European Federation of Societies for Ultrasound in Medicine and Biology formed a Gastrointestinal Ultrasound (GIUS) task force group to promote the use of GIUS in a clinical setting. One of the main objectives of the task force group was to develop clinical recommendations and guidelines for the use of GIUS under the auspices of EFSUMB. The first part, gives an overview of the examination techniques for GIUS recommended by experts in the field. It also presents the current evidence for the interpretation of normal sonoanatomical and physiological features as examined with different ultrasound modalities

EFSUMB Recommendations and Guidelines for Gastrointestinal Ultrasound - Part 1: Examination Techniques and Normal Findings (Long version).

Abstract ▼ In October 2014 the European Federation of Societies for Ultrasound in Medicine and Biology formed a Gastrointestinal Ultrasound (GIUS) task force group to promote the use of GIUS in a clinical setting. One of the main objectives of the task force group was to develop clinical recommendations and guidelines for the use of GIUS under the auspices of EFSUMB. The first part, gives an overview of the examination techniques for GIUS recommended by experts in the field. It also presents the current evidence for the interpretation of normal sonoanatomical and physiological features as examined with different ultrasound modalities

Mapping of a new locus for congenital anomalies of the kidney and urinary tract on chromosome 8q24

Background. Congenital anomalies of the kidney and urinary tract (CAKUT) account for the majority of end-stage renal disease in children (50%). Previous studies have mapped autosomal dominant loci for CAKUT. We here report a genome-wide search for linkage in a large pedigree of Somalian descent containing eight affected individuals with a non-syndromic form of CAKUT. Methods. Clinical data and blood samples were obtained from a Somalian family with eight individuals with CAKUT including high-grade vesicoureteral reflux and unilateral renal agenesis. Total genome search for linkage was performed using a 50K SNP Affymetric DNA microarray. As neither parent is affected, the results of the SNP array were analysed under recessive models of inheritance, with and without the assumption of consanguinity. Results. Using the non-consanguineous recessive model, a new gene locus (CAKUT1) for CAKUT was mapped to chromosome 8q24 with a significant maximum parametric Logarithm of the ODDs (LOD) score (LODmax) of 4.2. Recombinations were observed in two patients defining a critical genetic interval of 2.5 Mb physical distance flanked by markers SNP_A-1740062 and SNP_A-1653225. Conclusion. We have thus identified a new non-syndromic recessive gene locus for CAKUT (CAKUT1) on chromosome 8q24. The identification of the disease-causing gene will provide further insights into the pathogenesis of urinary tract malformations and mechanisms of renal developmen

Effect of dietary fatty acids on expression of lipogenic enzymes and fatty acid profile in tissues of bulls

This study investigated the effects of dietary linolenic acid (C18:3n-3) v. linoleic acid (C18:2n-6) on fatty acid composition and protein expression of key lipogenic enzymes, acetyl-CoA carboxylase (ACC), stearoyl-CoA desaturase (SCD) and delta 6 desaturase (6d) in longissimus muscle and subcutaneous adipose tissue of bulls. Supplementation of the diet with C18:3n-3 was accompanied by an increased level of n-3 fatty acids in muscle which resulted in decrease of n-6/n-3 ratio. The diet enriched with n-3 polyunsaturated fatty acids (PUFAs) significantly inhibited SCD protein expression in muscle and subcutaneous adipose tissue, and reduced the 6d expression in muscle. There was no significant effect of the diet on ACC protein expression. Inhibition of the 6d expression was associated with a decrease in n-6 PUFA level in muscles, whereas repression of SCD protein was related to a lower oleic acid (C18:1 cis-9) content in the adipose tissue. Expression of ACC, SCD and 6d proteins was found to be relatively higher in subcutaneous adipose tissue when compared with longissimus muscle. It is suggested that dietary manipulation of fatty acid composition in ruminants is mediated, at least partially, through the regulation of lipogenic enzymes expression and that regulation of the bovine lipogenic enzymes expression is tissue specific. © 2010 The Animal Consortium

Intercellular Diffusion of a Fluorescent Sucrose Analog via the Septal Junctions in a Filamentous Cyanobacterium

D.J.N. was supported by a Queen Mary University of London College studentship. M.N.M. was the recipient of an FPU (Formación del Personal Universitario) fellowship from the Spanish Government. Work in Seville was supported by grant BFU2011-22762 from Plan Nacional de Investigación, Spain, cofinanced by the European Regional Development Fund, and by Plan Andaluz de Investigación, Regional Government of Andalucía (grant P10-CVI-6665). Research in Tübingen was supported by the Deutsche Forschungsgemeinschaft (SFB766)

Duplications at 19q13.33 in patients with neurodevelopmental disorders

Objective After the recent publication of the first patients with disease-associated missense variants in the GRIN2D gene, we evaluate the effect of copy number variants (CNVs) overlapping this gene toward the presentation of neurodevelopmental disorders (NDDs). Methods We exploredClinVar (number ofCNVs = 50,794) andDECIPHER (number ofCNVs = 28,085) clinical databases of genomic variations for patients with copy number changes overlapping the GRIN2D gene at the 19q13.33 locus and evaluated their respective phenotype alongside their frequency, gene content, and expression, with publicly available reference databases. Results We identified 11 patients with microduplications at the 19q13.33 locus. The majority of CNVs arose de novo, and comparable CNVs are not present in control databases. All patients were reported to have NDDs and dysmorphic features as the most common clinical phenotype (N = 8/11), followed by seizures (N = 6/11) and intellectual disability (N = 5/11). All duplications shared a consensus region of 405 kb overlapping 13 genes. After screening for duplication tolerance in control populations, positive gene brain expression, and gene dosage sensitivity analysis, we highlight 4 genes for future evaluation: CARD8, C19orf68, KDELR1, and GRIN2D, which are promising candidates for disease causality. Furthermore, investigation of the literature especially supports GRIN2D as the best candidate gene. Conclusions Our study presents dup19q13.33 as a novel duplication syndrome locus associated with NDDs. CARD8, C19orf68, KDELR1, and GRIN2D are promising candidates for functional follow-up.Peer reviewe

Treatment of melanoma cells with the synthetic retinoid CD437 induces apoptosis via activation of AP-1 in vitro, and causes growth inhibition in xenografts in vivo

Human malignant melanoma is notoriously resistant to pharmacological modulation. We describe here for the first time that the synthetic retinoid CD437 has a strong dose-dependent antiproliferative effect on human melanoma cells (IC50: 5 x 10(-6) M) via the induction of programmed cell death, as judged by analysis of cell morphology, electron microscopical features, and DNA fragmentation. Programmed cell death was preceded by a strong activation of the AP-1 complex in CD437-treated cells as demonstrated by gel retardation and chloramphenicol transferase (CAT) assays. Northern blot analysis showed a time-dependent increase in the expression of c-fos and c-jun encoding components of AP-1, whereas bcl-2 and p53 mRNA levels remained constant. CD437 also exhibited a strong growth inhibitory effect on MeWo melanoma cells in a xenograft model. In tissue sections of CD437-treated MeWo tumors from these animals, apoptotic melanoma cells and c-fos overexpressing cells were colocalized by TdT-mediated deoxyuridine triphosphate-digoxigenin nick end labeling (TUNEL) staining and in situ hybridization. Taken together, this report identifies CD437 as a retinoid that activates and upregulates the transcription factor AP-1, leading eventually to programmed cell death of exposed human melanoma cells in vitro and in vivo. Further studies are needed to evaluate whether synthetic retinoids such as CD437 represent a new class of retinoids, which may open up new ways to a more effective therapy of malignant melanoma

Defective Gpsm2/G alpha(i3) signalling disrupts stereocilia development and growth cone actin dynamics in Chudley-McCullough syndrome

Mutations in GPSM2 cause Chudley-McCullough syndrome (CMCS), an autosomal recessive neurological disorder characterized by early-onset sensorineural deafness and brain anomalies. Here, we show that mutation of the mouse orthologue of GPSM2 affects actin-rich stereocilia elongation in auditory and vestibular hair cells, causing deafness and balance defects. The G-protein subunit Gαi3, a well-documented partner of Gpsm2, participates in the elongation process, and its absence also causes hearing deficits. We show that Gpsm2 defines an ∼200 nm nanodomain at the tips of stereocilia and this localization requires the presence of Gαi3, myosin 15 and whirlin. Using single-molecule tracking, we report that loss of Gpsm2 leads to decreased outgrowth and a disruption of actin dynamics in neuronal growth cones. Our results elucidate the aetiology of CMCS and highlight a new molecular role for Gpsm2/Gαi3 in the regulation of actin dynamics in epithelial and neuronal tissues